Figure 2.

HULC is not an interferon (IFN)–stimulated gene. A, Huh-7.5 cells were transfected with a plasmid encoding N-terminal FLAG tagged RIG-I or the corresponding empty vector, and 24 hours later, they were transfected with 1 μg/mL poly(I:C) or mock. Total RNA was extracted 9, 24, and 48 hours after poly(I:C) transfection. HULC and β-actin messenger RNA (mRNA) levels were determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR). HULC expression was normalized to β-actin mRNA expression, and the normalized RNA levels from poly(I:C)-transfected cells were further normalized to those from mock-transfected cells at each time point and plasmid transfection. B, Huh-7.5 cells were treated with IFN-α2b or IFN-λ3 at the indicated concentrations and total RNA was extracted. The mRNA levels of MX1, OAS2, and 18S ribosomal RNA (rRNA) were quantified by RT-qPCR, and MX1 and OAS2 mRNA levels were normalized to those of 18S rRNA. NT indicates nontreatment.