Fit results for the FCS data in Fig. 2b. The Alexa Fluor 647 concentration for the NR and ZMW is 2.8 μM, while it is 15 nM for the confocal case. The excitation power is 20 μW at 633 nm. F is the total fluorescence intensity and CRM = F/N is the fluorescence brightness per molecule. The background is neglected here as it is negligible as compared to the fluorescence signal.
| N | τ d (μs) | T ds | τ ds (μs) | F (kHz) | CRM (kHz) | |
|---|---|---|---|---|---|---|
| Confocal | 16 | 140 | 0.41 | 4 | 72 | 4.5 |
| ZWM 110 nm | 4.2 | 25 | 0.36 | 1.7 | 219 | 52 |
| NR 120 × 30 nm2 | 3.0 | 8.3 | 0.06 | 2 | 239 | 80 |