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. 2020 Jul 23;2(9):4153–4160. doi: 10.1039/d0na00366b

Fit results for the FCS data acquired on p-terphenyl in Fig. 4b. The concentration for the NR and ZMW is 10 μM, while it is 200 nM for the confocal case. The solvent is ethanol with 40% glycerol. The excitation power is 200 μW at 266 nm. Due to the larger noise on the FCS correlation, the triplet blinking (Tds and τds) is not considered in the fit.

N τ d (μs) F (kHz) B (kHz) CRM (kHz)
Confocal 290 250 56.2 0.4 0.2
ZWM 70 nm 8.7 86 10.3 1.5 1.0
NR 60 × 45 nm2 0.9 27 2.3 1.0 1.5