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. 2022 Aug 26;8(34):eabn3106. doi: 10.1126/sciadv.abn3106

Fig. 4. Lin28a overexpression abolished cartilage destruction and restored anabolism in OA.

Fig. 4.

(A) Schematic representation of regenerative protocol applied to Col2a1-CreER (CT) and Lin28aflox/flox/Col2a1-CreER [Lin28a(Tg)] mice. OA was induced in mice at age 10 weeks, then mice were euthanized at 4, 8, and 14 weeks after OA induction. (B) Safranin-O staining and quantification of OA score for articular cartilage in sham and OA CT and Lin28a(Tg) mice (scale bars, 100 μm). (C) Immunohistochemistry of MMP13 and quantification (scale bars, 100 μm). (D) Immunofluorescence of PRG4 and quantification (scale bars, 100 μm). (E) Osteophyte volume analyzed by microtomography. (F) Subchondral BV/TV analyzed by microtomography. Data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001.