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. 2022 Aug 26;8(34):eabn3106. doi: 10.1126/sciadv.abn3106

Fig. 6. HMGA2 expression increased anabolism and reduced catabolism in chondrocytes.

Fig. 6.

(A) Western blot analysis of HMGA2 protein expression in primary chondrocytes transduced with control empty vector [Ct(EV)] or Lin28a lentivirus [Lin28a(TG)] at 1% O2. (B) Immunofluorescence staining and quantification of HMGA2 expression in cartilage from mice (scale bars, 100 μm). (C) Western blot analysis of HMGA2, MMP13, and SOX9 protein levels in primary chondrocytes transduced with scramble vector (shSRC), empty vector [Ct(EV)], shRNA against HMGA2 (shHMGA2), or HMGA2 lentivirus [HMGA2(TG)] at 1% O2. (D) RT-qPCR analysis of HMGA2, MMP13, and SOX9 mRNA levels in primary chondrocytes treated with shSRC or shHMGA2 at 1% O2. (E) Alcian blue staining of sulfated glycosaminoglycans (GAGs) in chondrocytes treated or not with shHMGA2 or lentivirus. Wnt3a was used to induce chondrocyte catabolism (scale bars, 200 μm). Data are means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.005.