TABLE 1.
Primers used in this study
| Designation | Sequence (5′→3′)a |
|---|---|
| gapfor | GTAACGTATTCCGCGCAG |
| gaprev | CTAATACGACTCACTATAGGGAGAGCTGCAAGGTCAACAACG |
| fbaAfor | GCGATTCACTTAGACCACG |
| fbaArev | CTAATACGACTCACTATAGGGAGACGTGGATCGTACTCGTCAG |
| pgkfor | CGATACACGTATCCGTGC |
| pgkrev | CTAATACGACTCACTATAGGGAGACGTACCAATGTCGATTGC |
| enofor | GCACGCGAAGTATTAGACTC |
| enorev | CTAATACGACTCACTATAGGGAGAGGTCGTCACCAACAAGC |
| yvbQfor | CGAAGAAGCCTGTCTGC |
| yvbQrev | CTAATACGACTCACTATAGGGAGACGGTAAGTGCCTGAAGC |
| ackAfor | CGGTATCGCCGACAGCGTATTC |
| ackArev | CTAATACGACTCACTATAGGGAGAAACCGCGAAGAACGCGTTCTC |
| acsAfor | GAAGCGATTGACCGCCATGC |
| acsArev | CTAATACGACTCACTATAGGGAGATTCAATCGTTCCATACCAGC |
| alsSfor | CGTGATCCGTGCATATCG |
| alsSrev | CTAATACGACTCACTATAGGGAGAGGAGTGCGAACCGATATCGC |
| acuBfor | CGAAGACGGATACGCTTG |
| acuBrev | CTAATACGACTCACTATAGGGAGAGACGTGGTGTCCATTTC |
| citHfor | GACAGAGCTTTAGGATAATACTTTCATGACATTT |
| citHrev | TCACAGAGCAGAGAGACATGGGAAATACTC |
| sucDfor | GACAGAGCTTATTAATGCGTTTTACAAGTTTC |
| sucDrev | TCACAGAGCATGAGTGTTTTCATTAATAAAGATA |
a
T7 recognition sequences are underlined.