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. 2019 Oct 18;2(1):17–36. doi: 10.1039/c9na00583h

Singlet oxygen (1O2) detection methods.

Method Probe Detection
Phosphorescence 1O2 Phosphorescence of 1O2 at 1270 nm
UV-vis spectroscopy 9,10-Diphenyl-anthracene (DPA) Decrease peak at 355 nm
UV-vis spectroscopy 9,10-Anthracenediyl-bis(methylene) dimalonic acid (ABDA) Decrease peak at 382 nm
UV-vis spectroscopy 9,10-Anthracenedipropionic acid (ADPA) Decrease peak at 400 nm
UV-vis spectroscopy Anthracene-9,10-bisethanesulfonic acid (AES) Decrease peak at 360, 378 and 400 nm
UV-vis spectroscopy 1,3-Diphenyl-isobenzofuran (DPBF) Decrease peak at 410 nm
UV-vis spectroscopy p-Nitrosodimethylaniline imidazole (RNO method) Decrease peak at 440 nm
Fluorescence Singlet oxygen sensor Green (SOSG) Increased fluorescence at 540 nm
Fluorescence Methotrexate (MTX) Increased fluorescence at 450 nm
ESR 2,2,6,6-Tetramethyl-piperidine (TEMP) Change in enhanced permeability and ESR spectrum