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. 2020 Mar 31;2(5):2025–2033. doi: 10.1039/d0na00141d

Fig. 3. (A) The Raman spectra from the multidrug resistant strain of Salmonella DT104 and the normal strain of Salmonella Typhi on the heterostructure surface. To remove the Eg and A1g band contributions from WS2, we subtracted the Raman spectra of the pathogens with the heterostructure SERS substrate from the spectrum for the heterostructure SERS substrate on its own. (B) Plots showing how the Raman spectrum from the multidrug resistant strain of Salmonella DT104 varies with concentration (CFU mL−1). (C) Plots showing how the Raman spectrum from the normal strain of Salmonella Typhi varies with concentration (CFU mL−1). (D) Plots showing how the time dependent Raman spectra from the normal strain of Salmonella Typhi vary during Augmentin antibiotic treatment. We used 1000 CFU mL−1Salmonella Typhi bacteria for this experiment. (E) A plot showing how the time dependent Raman spectra from the multidrug resistant Salmonella DT104 vary during Augmentin antibiotic treatment We used 1000 CFU mL−1Salmonella DT104 bacteria for this experiment. (F) A SEM image showing that the bacterial cell wall gets damaged when the normal strain of Salmonella Typhi was treated with the Augmentin antibiotic. (G) A SEM image of the Salmonella DT104 bacteria after antibiotic treatment. It shows that the bacterial cell wall remains intact when multidrug resistant Salmonella DT104 was treated with the Augmentin antibiotic. (H) Plots indicating the percentages of live bacteria for Salmonella DT104 and Salmonella Typhi during treatment with the Augmentin antibiotic. We used 1000 CFU mL−1 bacteria for this experiment. (I) A plot showing how the SERS intensity at 1140 cm−1 decreases as the number of live bacteria decreases during treatment with the Augmentin antibiotic. We reported the ratio of SERS intensity at 1140 cm−1 before and after treatment. We used 1000 CFU mL−1Salmonella Typhi bacteria for this experiment. (J) Plots showing the relative cellular ATP leakage from Salmonella DT104 and Salmonella Typhi after treating with the Augmentin antibiotic.

Fig. 3