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Construction of AKP5 strain with des under spac promoter control. Plasmid pPA13, which contains the 28-nucleotide-long 5′ UTR and the first 147 codons of the des gene downstream of the spac promoter, was used to transform wild-type strain JH642 to Cmr, yielding strain AKP5. Campbell insertion of this plasmid places the des gene under the control of the spac promoter.
