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. 1999 Nov;181(22):7028–7033. doi: 10.1128/jb.181.22.7028-7033.1999

FIG. 6.

FIG. 6

des mRNA production under the control of the spac promoter. Total RNA (10 μg/ml) from strain AKP5 (lanes 1 to 4) or JH642 (lane 5) was separated on formaldehyde-agarose gels. Strain AKP5 was grown at 37°C to mid-exponential phase, and half of this culture was supplemented with 1 mM of IPTG. Treated (+) and untreated (−) cultures were further shifted to 20°C for 45 min (lanes 1 and 2) or maintained at 37°C for 15 min (lanes 3 and 4). Strain JH642 was grown at 37°C to mid-exponential phase and then shifted to 20°C for 45 min (lane 5).