FIG. 8.

Pattern of spac-des expression at 20°C. Total RNA (10 μg/ml) from strain AKP5 was separated on formaldehyde-agarose gels. Cells were grown at 37°C to mid-exponential phase, supplemented with 1 mM IPTG, and then shifted to 20°C. Total RNA was extracted after the addition of IPTG (times shown indicate hours elapsed following addition of IPTG).