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. 2022 Aug 17;21:97–109. doi: 10.1016/j.bioactmat.2022.08.005

Fig. 6.

Fig. 6

Effect of gel-scaffold constructs on angiogenesis of HUVECs. (a) The viability of HUVECs treated with the extracts of gel-scaffold constructs for 1, 3 and 5 days. (b) mRNA expression of angiogenesis-related genes (VWF, CD31 and PDGF-B) of HUVECs cultured for 3 days. (c) Immunofluorescence images of HUVECs, the red fluorescent area represented the VWF protein, and the blue fluorescent area represented the nuclei. (d) Bright-field optical microscopy observation of tube formation of HUVECs. (e) Fluorescence images of HUVECs stained with Calcein-AM. (f) Number of tubes formed by HUVECs. *p < 0.05, **p < 0.01, ***p < 0.001.