Fig. 9.

Expression of matrix and marker proteins in the bone defect after gel-scaffold construct implantation. (a) Masson's trichrome staining images of cranial defects after the implantation of gel-scaffold constructs for 4 and 8 weeks, the NV indicated by the arrow represented for new blood vessels, and NB represented for new bone tissue. (b) OCN and CD31 immunohistochemical staining images of cranial defects after the implantation of gel-scaffold constructs for 4 weeks, the yellow-brown area was the positive expression area of OCN and CD31. (c) CD31 immunofluorescence staining images of cranial defects after the implantation of gel-scaffold constructs for 4 weeks, CD31 was marked in red and the nuclei were marked in blue. (d) Quantitative analysis of newly formed micro-vessels in each group based on CD31 immunofluorescence staining.