Fig. 11. Determination of cell death and apoptosis of brain microvascular endothelial cells after treatment with MNPs for 24 h (top) and 72 h (bottom). Cells were analyzed by flow cytometry after staining with annexin-V-FITC and propidium iodide. Cell populations in percent of total cells have been blotted. One-way ANOVA followed by Tukey's multiple comparisons test was performed. The data of each group (living, dead, and apoptotic) were statistically evaluated separately from each other (n = 3–4).