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. 2022 Aug 18;7(9):1390–1403. doi: 10.1038/s41564-022-01195-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Heterologous expression of cholesterol sulfotransferase candidates in BL21 E. coli treated with either Chol^Alk or cholesterol and ion chromatograms representing the detection of Chol^Alk-Sulf or Chol^Sulf, respectively (n = 2 biological replicates per gene candidate, representative traces are shown here) b, In vitro conversion of cholesterol to Chol^Sulf and Chol^Alk to Chol^Alk-Sulf via enriched His₆_BT_0416 (n = 2 biological replicates per condition, representative traces are shown here) c, Ion chromatograms demonstrating that the Bt_0416 deletion mutant does not make Chol^Sulf. d, Putative biosynthesis of PAPS followed by the biosynthesis of Chol^Sulf in Bacteroides. Gene products are coloured according to the key in e. e, Biosynthetic clusters representing putative Chol^Sulf biosynthesis genes in B. thetaiotaomicron, B. ovatus and B. fragilis. Chromatograms are scaled to the largest peak in each dataset. The red text highlights metabolic functions attributed to BT_0416.

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