FIG. 5.

Western blot analysis of spore coat extracts. Coat protein extracts were electrophoresed on 15% polyacrylamide gels and transferred to polyvinylidene difluoride membranes. CotE was detected with anti-CotE antibodies. Extracts in each lane were prepared from spores from the indicated strain. The positions of CotE are indicated by triangles. Bands below CotE are likely to be degradation products. The difference in migration between CotE in spores from PY79 and AD408 and the similarity in mobility of PY79 and TB50 are due to the additional mass of the HA1 epitope. Molecular masses are indicated in kilodaltons.