TABLE 2.

Primers

Oligonucleotide	Sequencea	Enzyme	Nucleotidesb
OL3	5′ AAAAAGAATTCAGCTCGTTGCACACACC 3′	EcoRI	−386 to −370
OL4	5′ AAAAAGGATCCTACTGTCTCCCCTAGTCCC 3′	BamHI	+551 to +569
OL22R	5′ AAAAACTCGAGTTCTTCAGGGCTGGCGTAGT 3′	XhoI	+536 to +544
OL23	5′ AAAACTCTTCAGAAAAAAAACCGAGCAGC 3′	Eam1104I	+85 to +102
OL24	5′ AAAACTCTTCAGAAATTGAAGGGTATTAT 3′	Eam1104I	+163 to +180
OL25	5′ AAAACTCTTCATTCAGACATTCCGGCATG 3′	Eam1104I	−9 to +9
OL26R	5′ AAAACTCTTCAGAAGATGAGCTTGATGAA 3′	Eam1104I	+478 to +495
OL27	5′ AAAACTCTTCATTCAACAACTACCTTTGTTTCCCC 3′	Eam1104I	+415 to +438
OL31	5′ AAAAAAAACATATGTCTGAATACAGGGAAATTAT 3′	NdeI	+1 to +23
OL35R	5′ AAAAAGGATCCTTAGGCGTAGTCGGGGACGTCGTAGGGGTAAACTACCTTTGTTTCCCC 3′	BamHI	+415 to +432
OL36	5′ AAAAAGGATCCTTAGGCGTAGGACGTCGTAGGGGTAATCTTCCTCGTCATCCTC 3′	BamHI	+457 to +474
OL37	5′ AAAAAGGATCCTTAGGCGTAGGACGTCGTAGGGGTACGGGTTGATGTCTTCAAGCTC 3′	BamHI	+496 to +516
OL41	5′ AAAACTCTTCATTCTGTGACAACCTCTGT 3′	Eam1104	+220 to +237
OL42	5′ AAAACTCTTCAGAAGTGATTGCCAAAGTG 3′	Eam1104I	+304 to +321
OL49	5′ AAAACTCTTCATTCAGGCGAGATGGT 3′	Eam1104I	+73 to +87
OL50	5′ AAAACTCTTCATTCAATTTCTACCGTTTTTCC 3′	Eam1104I	+151 to +171
OL51R	5′ AAAACTCTTCAGAAGTTGTCACAGAACGG 3′	Eam1104I	+223 to +240

^aThe restriction endonuclease site in each oligonucleotide sequence is underlined, and the enzymes that cut them are listed. 

^bNucleotide positions are relative to the start of translation of cotE (34, 35) and indicate the region of the chromosome bound by each oligonucleotide.