Fig. 5. Site-specific functionalization of different surface nanostructures made by colloidal lithography. (a–c) Unspecific functionalization with thiols uniformly deposits gold nanoparticles onto the entire surface of the nanostructure, exemplarily demonstrated for (a) single nanocrescents, (b) nanodisks, and (c) nanoholes. (d–f) Modification of the fabrication process by adding a passivation layer under a different deposition angle selectively uncovers specific parts of the nanostructure and provides a tool to direct the deposition of the nanoparticles onto predefined areas. (d) Rotated evaporation of the passivation layer selectively blocks one tip of a nanocrescent, enabling the formation of structures with a chiral character upon selective adsorption of nanoparticles. (e) Direct evaporation of the passivation layer onto nanodisks blocks the top surface and allows selective functionalization of the edge of the disc. (f) Azimuthal rotation of the passivation layer on top of a gold nanohole arrays enables an asymmetric functionalization of the array with AuNPs.