Fig. 2.

Representative LC-MS/MS chromatograms of sterol standards (A) and extracted sterols from Leishmaniadonovani LV82 promastigote parasites treated with the vehicle (B), posaconazole (C), or fluconazole (D). Corresponding MRM transitions and retention times can be found in Table 1. LV82 promastigotes were treated with 0.4% DMSO vehicle, 20 μM PSZ, or 50 μM FLU. Arrows indicate the LC retention time of corresponding sterol standards.