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. 2022 Aug 15;20:27–42. doi: 10.1016/j.ijpddr.2022.07.003

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Structural determination of the purified intermediate sterol 4,14-dimethylzymosterol. (A) The exact mass of the dehydrated precursor ion was determined by high resolution TOF mass spectrometry. (B–G) The 1D and 2D NMR spectra of lanosterol (reference) and the purified intermediate sterol. ¹H spectra (B), DEPT (C–E), HMBC (F, G), and NOESY (H, I). 4,14-Dimethylzymosterol is referred to as “unknown” in the figures.

Fig. 5 — Structural determination of the purified intermediate sterol 4,14-dimethylzymosterol. (A) The exact mass of the dehydrated precursor ion was determined by high resolution TOF mass spectrometry. (B–G) The 1D and 2D NMR spectra of lanosterol (reference) and the purified intermediate sterol. ¹H spectra (B), DEPT (C–E), HMBC (F, G), and NOESY (H, I). 4,14-Dimethylzymosterol is referred to as “unknown” in the figures.