FIG. 7.

Wild-type and truncated Rap1p binding to CAR2 and TEF2 DNA fragments. DNA fragments ³²P labeled at their 5′ ends and containing the HD190 and HD192 sequences indicated in Fig. 1 were used as probes. Yeast cell extract (EXT) was omitted from the reaction mixtures resolved in lanes A and E. A wild-type (strain W303-1A) extract (W.T.) was used in lanes B, D, F, and H. An extract from a rap1 truncation mutant strain (mut.) (YLS91) was used in lanes C and G (rap1Δ).