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. 2022 Aug 26;12:14570. doi: 10.1038/s41598-022-18779-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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N-glycosidase F effect on CD98. Protein extracts of HEK293 cells transiently transfected with WT-CD98 or mutant-CD98 constructs were subjected to western blot before (upper panel) or after (lower panel) treatment with N-glycosidase F for 3 h at 37 °C. Western blot analysis was carried out using the anti-FLAG antibody³³. Loading order: WT, N365Q, N381Q, N424Q, N506Q, Dm (double mutant: N381/424Q), Tm (triple mutant: N365/381/424Q), Qm (quadruple mutant: N365/381/424/506Q). The western blot image is representative of three independent experiments.