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. 2022 Aug 26;12:14570. doi: 10.1038/s41598-022-18779-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell surface biotinylation of CD98. Surface protein fractions isolated as described in material and methods were entirely loaded on SDS-PAGE for western blot analysis; total lysate used to perform membrane protein fraction isolation was shown as a control. Loading order: Qm total lysate (lane1), WT total lysate (lane 2), Qm surface fraction (lane 3) and WT surface fraction (line 4). The western blot was performed using anti-FLAG³³. The image is representative of three independent experiments. The histogram represents mean of values obtained by scanning densitometry of immunoblots. Statistical analysis was performed for each construct measuring membrane fraction with respect to the total lysate by Student's t-test.