Skip to main content
. 2022 Aug 26;12:14607. doi: 10.1038/s41598-022-18025-x

Figure 1.

Figure 1

3DULM for in vivo angiography at the early phase of stroke’s models. (a) Skin opening for ultrasound imaging. Ischemic model: a small hole was drilled above the MCA branch, and human thrombin was injected. Hemorrhagic model: the parietal bone was drilled, and collagenase was injected in the striatum. (b) Timeline of the experiment for each model, with four acquisitions: in the half hour prior stroke onset (t−), in hyperacute stage (30 min and 1h30), and the day after with MRI imaging. T2* and T2 MRI sequences slices and 3D rendering. (c) Ultrasound matrix probe with transducers pooled in four synthetic apertures successively connected to the echograph. ULM processing with filtering, localization and tracking. Rat brain atlas registered on ULM volume (600 µm width slab). 3D rendering with brain surface with Amira software (Thermo Fisher). Scale bars: 1 mm. Figures were created with (a–c) Illustrator 2021 (https://www.adobe.com/products/illustrator), (c) MatLab 2019a (https://www.mathworks.com/products/matlab), (c) Amira 2019.4 (https://www.thermofisher.com/amira-avizo).