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. 2022 Aug 26;12:14607. doi: 10.1038/s41598-022-18025-x

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MDI for unsupervised observation reflects the vascularization density. (a) Anatomical regions are extracted from anatomical structures. The hidden areas are excluded from the study. MDI computation with a range of 100 µm on binarized vascularization. (b) Selection of a round reference region of 1.2 mm diameter in the thalamus and a region of interest in the cortex for ischemic stroke (0.9 mm diameter) and in the striatum for hemorrhage. (c) Example of a spherical ROI extracted from the ULM volume, binarization and MDI computation. Figures were created with (a,b) MatLab 2019a (https://www.mathworks.com/products/matlab), (c) Amira 2019.4 (https://www.thermofisher.com/amira-avizo).