Table 3.
Comparison of fermentation parameters in the culture media and vitamin B12 production by P. freudenreichii DSM 20271. A, B, C, D, E, F — homogeneous groups of the influence of the type of medium on the production of vitamin B12, the Tukey’s test (one-way analysis of variance)
| Medium | PA yield [g/g] | PA productivity [g/L/h] | AA yield [g/g] | AA productivity [g/L/h] | P/A ratio [g/g] | Vitamin B12 [µg/100 g wet biomass] |
|---|---|---|---|---|---|---|
| 1 | 0.14 | 0.017 | 0.06 | 0.007 | 2.26:1 | 126.58 ± 2.93A |
| 2 | 0.45 | 0.017 | 0.18 | 0.007 | 2.54:1 | 159.94 ± 12.29B |
| 3 | 0.00 | 0.000 | 0.00 | 0.000 | – | –D |
| 4 | 0.41 | 0.034 | 0.17 | 0.014 | 2.42:1 | 289.80 ± 9.68E |
| 5 | 0.12 | 0.014 | 0.03 | 0.004 | 3.69:1 | 90.80 ± 5.80C |
| 6 | 0.42 | 0.053 | 0.11 | 0.013 | 4.11:1 | 160.65 ± 13.63B |
| 7 | 0.48 | 0.068 | 0.13 | 0.018 | 3.68:1 | 100.50 ± 4.03C |
| 8 | 0.31 | 0.056 | 0.10 | 0.018 | 3.10:1 | 121.94 ± 6.73A |
| 9 | 0.30 | 0.027 | 0.11 | 0.011 | 2.51:1 | 242.31 ± 2.17F |
| 10 | 0.35 | 0.050 | 0.09 | 0.013 | 3.73:1 | 138.33 ± 11.58AB |
PA, propionic acid; AA, acetic acid; P/A, propionic acid/acetic acid weight ratio