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. 2022 Aug 26;13:5026. doi: 10.1038/s41467-022-32763-6

Fig. 3. BRAT1 deletion disrupts the structural integrity of Cajal bodies.

Fig. 3

a Immunofluorescence staining of subnuclear structures in U2OS and BRAT1−/− (clone #8) cells transfected with control non-targeting siRNA (siNT) or siINTS11, as indicated. Cajal bodies were visualized by immunostaining of coilin (green), nucleoli by B23 (red), and nuclei by DAPI (blue). b Quantification of normalized coilin fluorescence inside the nucleolus using ScanR software; representative pictures are shown in a. Data are represented as the mean ± SD (n = 3). Statistical significance was determined by a one-sided paired Student’s t-test (*p < 0.05, ***p < 0.001). See also Supplementary Fig. 3b, c.