Table 2.
The characteristics comparison of different DNA amplification approaches between LAMP assay in this study, lateral flow assay35 and electrochemical detection34 for detecting Leishmania DNA in human and animal, respectively.
| Characteristics | DNA amplification approaches | ||
|---|---|---|---|
| Lateral flow assay | Electro-chemical detection | LAMP assay in this study | |
| Isothermal amplification | + | + | + |
| Qualitative examination | |||
| Color interpretation by naked eye | + | − | + |
| Fluorescent interpretation by naked eye | − | − | + |
| Electrochemical detection by electronic device | − | + | − |
| Quantitative examination | |||
| Naked eye | − | − | + |
| Detecting device | + | + | + |
| Detection limits (parasites/mL) | 10 | 0.8 | 102 |
| Linear range (parasites/mL) | 0.04–20 | 0.5–500 | 103–106 |