Effects of THC treatment on G-CSF, BDNF, and GDNF protein concentrations in brain regions following CCI and sham surgery. X-axis=brain regions, and Y-axis=tissue concentration of the protein in pg/mg (A–C). G-CSF protein levels were significantly increased by 3 days of THC treatment in the sham surgery control group (A). Following CCI, G-CSF levels were significantly increased on day 7 and 14 in cortex and striatum, but not hippocampus (B, C). (D–F) BDNF levels were increased in both the sham control group (D) and following CCI on days 7 and 14 (E, F). (G–I) THC treatment resulted in increased GDNF levels in both the sham control group (G) and on day 7 and 14 after CCI in animals treated with THC compared to vehicle (H, I). Data analysis: 2-way ANOVA was performed for data set on each graph revealing that both time and brain region contributed significantly to total variance. Sidak's correction for multiple comparisons was performed to detect significant differences indicated by *p<0.05). BDNF, brain-derived neurotrophic factor; GDNF, glial-derived neurotrophic factor.