FIG. 5.

RT-PCR transcript analysis of the mercury resistance determinant and control 16S rRNA with total cellular RNA from B. cereus RC607 and E. coli JM109(pYW33), UI or I by growth with HgCl₂ for 2 h. (A) Mercury resistance genes. RT primers and subsequent PCR primers are indicated for each reaction, as are the sizes of the PCR products detected. (B) Control 16S rRNA gene RT-PCR from the same reactions as with merA or merB1 primers. A quantitative analysis of the ethidium bromide-stained PCR products is shown in Table 1.