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. 2022 Jul 19;298(9):102282. doi: 10.1016/j.jbc.2022.102282

Figure 7.

Figure 7

Stable-5-LOX activity with and without preincubation with the substrate analog 13-Hpode. All enzyme concentrations were 250 nM and 13-Hpode concentration was 2.5 μM; each concentration of AA was tested in triplicate. A, absorbance at 235 nm versus time plot; linear portion shown as red. AA concertation was 21.3 μM. B, saturation curves for Stable-5-LOX in the presence and absence of 13-Hpode. The embedded figure is given to show the shape of the curve in the presence of 13-Hpode. 5-LOX, 5-lipoxygenase; 13-Hpode, 13-hydroperoxy-9Z, 11E-octadienoic acid; AA, arachidonic acid.