Fig. 1.

Macrophage SAMSN1 expression is increased and negatively correlates with LPS-induced ALI in mice. (A) Mice were intratracheally injected with LPS (5 mg/kg) and sacrificed after 12 h, and then SAMSN1 protein level was detected in AMs isolated from mice with saline or LPS injection. (B) Correlation between SAMSN1 expression in AMs and tidal volume in LPS-injected mice (n = 20). (C) SAMSN1 protein level was detected in PBMCs isolated from mice with saline or LPS injection. (D) Correlation between SAMSN1 expression in PBMCs and tidal volume in LPS-injected mice (n = 20). (E–G) LPS-injected mice were divided into low-SAMSN1 or high-SAMSN1 groups using the median ratio of SAMSN1 expression in PBMCs as a threshold. LDH activity, PaO₂, PaCO₂ and HCO₃⁻ were detected. n = 6 per group, differences with P value < 0.05 were considered statistically significant.