Fig. 2.

Macrophage SAMSN1 deficiency exacerbates LPS-induced ALI in mice. (A) Samsn1 mRNA levels were detected in AT1, AT2 and AMs isolated from Samsn1^Flox or Samsn1^MKO mice. (B) SAMSN1 protein level was detected in AMs isolated from Samsn1^Flox or Samsn1^MKO mice. (C) Samsn1^Flox or Samsn1^MKO mice were intratracheally injected with LPS (5 mg/kg) and sacrificed after 12 h, and then LDH activity in the lungs was detected. (D) Lung wet to dry ratio. (E) Histopathological alterations of the lungs. (F–H) Pulmonary function was evaluated by airway resistance, lung compliance and tidal volume. (I–J) Blood gas analysis was determined by PaO₂, PaCO₂ and HCO₃⁻. (K) Protein concentrations in BALF. (L) Survival rate (n = 20). n = 6 per group, differences with P value < 0.05 were considered statistically significant.