Fig. 5.

Macrophage SAMSN1 overexpression ameliorates LPS-induced inflammation and oxidative stress in mice. (A) Samsn1^WT or Samsn1^MTG mice were intratracheally injected with LPS (5 mg/kg) and sacrificed after 12 h, and then the numbers of total cells, neutrophils and macrophages in BALF were detected. (B) MPO activity was detected to evaluate infiltration of neutrophils in the lungs. (C) The levels of TNF-α and IL-6 in the lungs. (D–E) The levels of ROS, MDA and 4-HNE. (F) Total SOD activity and GSH level. (G) NLRP3 and ASC protein levels. (H) Quantification of caspase-1 activity. (I–J) The levels of IL-1β and IL-18 in the lungs. n = 6 per group, differences with P value < 0.05 were considered statistically significant.