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. 1999 Nov;181(22):7098–7106. doi: 10.1128/jb.181.22.7098-7106.1999

FIG. 5.

FIG. 5

The right flank of the ery gene cluster contains two overlapping transcripts from eryBIV to eryBVII. Lane 1, Full-length eryBVI probe treated with S1 nuclease; lane 2, same as lane 1 but not treated with S1 nuclease; lane 3, 40 μg of yeast RNA hybridized with probe; lane 4, 40 μg of S. erythraea CA340 RNA hybridized with probe; lane 5, 40 μg of eryBIV::trrn RNA hybridized with probe; lane 6, 40 μg of CA340 eryAIII::trrn RNA hybridized with probe. All samples were treated with 50 U of S1 nuclease. A total of 3 × 104 Chelenkov counts per min were used per reaction. P, full-length protected fragment; the asterisk marks a shortened S1-protected fragment.