TABLE 2.
S. erythraea mutant strains generated by insertional mutagenesis or alteration of the −10 promoter region
| Straina | Gene Disruptedb | Phenotypec |
|---|---|---|
| eryAI::trrn | eryAI | Null |
| CA340eryAI::trrn | eryAI | Null |
| eryAIII::trrn | eryAIII | Null |
| CA340eryAIII::trrn | eryAIII | Null |
| eryBIII::trrn | eryBIII | 6-dEB |
| eryBIV::trrn | eryBIV | EB |
| eryBV::trrn | eryBV | EB |
| eryBVI::trrn | eryBVI | EB |
| eryCIV::trrn | eryCIV | EB/MEB |
| eryCVI::trrn | eryCVI | EB/MEB |
| ARR50d | eryAI | Null |
a
All strains are S. erythraea NRRL2338 unless listed as S. erythraea CA340.
b
Indicates gene containing an inserted 227-bp rrn operon terminator cassette by gene replacement.
c
Indicates the accumulation of the erythromycin intermediate(s) or the lack of production (null) of any erythromycin intermediate as assayed by TLC.
d
Contains only an altered eryAI −10 hexamer by gene replacement.