Fig. 5.

Knockout of CXCR2 attenuates inflammatory cell infiltration induced by Ang II.

(A) Wild-type (WT) and CXCR2 KO mice were infused with Ang II (3000 ng/min/kg) or saline for 3 weeks. Flow cytometry analyses of CD45⁺ cells, CD45⁺ CD11b⁺ F480⁺ macrophages and CD45⁺ CD11b⁺ Gr-1⁺ neutrophils cells in retinas (left). The percentage of gated cells among total cells (right, n = 6). (B) Representative images of Iba1 IHC staining, red arrows indicate the Iba1-positive microglia/macrophages(scale bar: 50 μm, left); quantification of positive cells in each group (right, n = 6). (C) qPCR analyses of the mRNA levels of IL-1β, IL-6, TNF-α, and MCP-1 in the retinas (n = 6). GAPDH was used as an internal control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)