Fig. 1. General overview of the study. (a) Myogenic differentiation process. Muscle precursor cells and myoblasts were fused to form immature myotubes, while the myogenin (Myog) expression increased. Myotube maturation implied an upregulation of Myosin Heavy Chain 2 (Myh2) and tropomyosin (Tnnt1) genes. (b) Principle of the cryogelation technique. (c) Protocol to generate a highly anisotropic cryogel.
