Fig. 7. Electrical Pulse Stimulation (EPS) enhances myogenic maturation. (a and b) Confocal merged images showing cells marked with phalloidin in red, cell nuclei counterstained in blue with DAPI and Mhc marked with Alexa-488 in green. Scale bars = 100 μm. (c) Graphs showing fusion index of CNTs(−) cryogels in light grey and CNTs(+) cryogels in dark grey. (d–f) Graphs of myogenic maturation marker expression. EPS(−) in light grey and EPS(+) in dark grey. (d) Myogenin. (e) Myosin heavy chain 2. (f) Troponin T1. Results are mean ± SEM. *p-value = 0.05.