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. 2022 Aug 5;64:101559. doi: 10.1016/j.molmet.2022.101559

Figure 2.

Figure 2

Recovering the DNMT3A expression in βRapKO partially restored the transcriptome and enhanced GSIS ex vivo. (A) Immunoblot analysis of DNMT3A expression in islets of 8-week-old WT + LvGFP, βRapKO + LvGFP and βRapKO + LvDnmt3a. (B) Principal component analysis (PCA) analysis was based on the transcriptome. Each dot represented an individual. The first three components were plotted in X, Y and Z axis. (C) GO analysis of differentially expressed genes as identified by RNA-seq analysis of 8-week-old βRapKO + LvGFP and βRapKO + LvDnmt3a islets. (D) Heatmap of selected genes in 8-week-old WT + LvGFP, βRapKO + LvGFP and βRapKO + LvDnmt3a. (E) Heatmap of selected immature genes and disallowed genes in islets of WT + LvGFP, βRapKO + LvGFP and βRapKO + LvDnmt3a. (F) Ex vivo GSIS experiments in WT + LvGFP, βRapKO + LvGFP and βRapKO + LvDnmt3a islets were performed. Islets were incubated with Krebs–Ringer bicarbonate buffer for 1-h and stimulated with 2.8 mM and 16.7 mM glucose for 1-h. Secreted insulin was normalized to total insulin in islets. Results were presented as mean ± SEM of independent experiment indicated as above. ∗P < 0.05, Differences between groups were assessed by ANOVA.