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. 2022 Aug 27;13:5054. doi: 10.1038/s41467-022-32579-4

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Live cell imaging of stage I-V gametocyte transfectants expressing PfNDC80-mCherry (NDC80-mCh, kinetochore marker; red), GFP-PfCENH3 (GFP-CENH3; CENH3; centromere marker; green) and counterstained with Hoechst (chromatin; grayscale) and Tubulin Tracker (TT, microtubules; cyan). b Contour length of the fluorescence profiles for PfNDC80-mCherry and nuclear microtubules (nuclear MT, marked by Tubulin Tracker with yellow arrows in Fig. 4a) (mean ± SD is shown, n = 20 cells). Statistical difference was determined by two-way ANOVA Tukey’s test. I: NS > 0.9999; II: ****p < 0.0001; IIIa: *p = 0.0132; IIIb: NS = 0.1481; IV: NS = 0.2092; V: NS = 0.8042. c Maximum length for the GFP-PfCENH3 and Hoechst labelling profiles (mean ± SD is shown, n = 20 cells). Statistical difference was assessed by two-way ANOVA Tukey’s test. I: NS < 0.9999; II: **p = 0.0011; IIIa: ****p < 0.0001; IIIb: NS = 0.0633; IV: NS = 0.5673; V: NS = 0.5106. d Maximum distance between the Pfcentrin-4-mCherry (Cen-4) and the GFP-PfCENH3 features (mean ± SD is shown, n = 20 cells per stage) in the Pfcentrin-4-mCherry/GFP-PfCENH3 co-transfectants. Statistical difference was analyzed by paired one-way ANOVA Tukey’s test. I vs. II: ****p < 0.0001; II vs. IIIa: NS = 0.2030; IIIa vs. IIIb: ****p < 0.0001; IIIb vs. IV: ***p = 0.0004; IV vs. V: NS = 0.6631. e Live cell imaging of Pfcentrin-4-mCherry (Cen-4-mCh, red) and GFP-PfCENH3 (GFP-CENH3, green) transfectants. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images and label pairings are presented in Supplementary Fig. 10. Source data for Fig. 4b–d is provided in the Source Data file.