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Fig. 2. Mi-Exo characterization. (A) Cryo-EM image of Mi-Exo. Cyan and orange triangles indicate the bi-lipid layer of Mi-Exo. (B) Western blotting. Mi-Exo marker (TSG101) and bacterial OMV marker (OmpF). (C) Bta-MiR-2478 detection using Real-Time PCR (RT-PCR). Grey line indicates negative controls (primer set and eOMVs). (D) Mi-Exo stability test on storage at 4 °C (Size, Ct value of Bta-MiR-2478 using RT-PCR) for day 1, 15, and 30. All results were measured at least three times.