TABLE 1.
Properties of the recovered plasmids
| Plasmida | Duplicated target sequence | % G+Cb | Accession no.c | Similarity to known sequenced |
|---|---|---|---|---|
| pWW1 | GTCTCATC | 55 | AF150937 | H. salinarum pHH1 plasmid ORF H0337 (signal-transducing histidine kinase); AAC82814 (E = 1.2 × 10−6) |
| pWW2 | AACGGTTT | 54 | AF150938 | Conserved hypothetical protein in Pyrococcus horikoshii; BAA29456 (E = 0.005) |
| pWW3 | ATCGGGAT | 41 | AF150939 | ISH50; X01584, AF016485 |
| pWW4 | ATCTGATC | 52 | AF150940 | |
| pWW5 | GTCTCATTC | 46 | AF150941 | |
| pWW6 | AAATAATA | 51 | AF150942 | H. salinarum pHH1 plasmid ORF H0761 (putative cell division control-related protein); AAC82854 (E = 2 × 10−6). Conserved hypothetical protein of Archaeoglobus fulgidus; AAB90352 (E = 2 × 10−4) |
| pWW7 | AAATGAAG | 51 | AF150943 | |
| pWW8 | CTCGGGAG | 63 | AF150944 | Candida parapsilosis (mitochondrion) cytochrome c oxidase III; Q34214 (E = 4 × 10−8) |
| pWW9 | GTTGAGTC | 60 | AF150945 | |
| pWW10 | GGGAGAGGG | 60 | AF150946 | |
| pWW11 | GACTTGTT | 52 | AF150947 | ISH27, ISH51; X54432 (E = 8 × 10−65), X54433, X54434, X04389, AF016485 |
| pWW12 | GATCATTG | 61 | AF150948 | |
| pWW13 | GACGGTAT | 51 | AF150949 | |
| pWW14 | CTCCTGTT | 53 | AF150950 | Archaeoglobus fulgidus, type II secretion system; AAB90245 (E = 0.001) |
| pWW15 | CCTATTTC | 54 | AF150951 | Dehydrogenase-oxidoreductase; S70672, D1031947 (E = 5 × 10−5) |
| pWW16 | GTCTCGTC | 46 | AF150952 | |
| pWW17 | CGTGCGTT | 58 | AF150953 | |
| pWW18 | GTTGAATC | 61 | AF150954 | |
| pWW19 | GTAAATAG | 48 | AF150955 | |
| pWW20 | GGCAAATC | 42 | AF150956 |
a
Plasmids recovered in E. coli from the DNA of H. hispanica::ThD73 transformants.
b
The percentage of guanine and cytosine content over the region sequenced (approximately 400 to 500 bp surrounding each target site).
c
The GenBank accession number for each flanking sequence.
d
Similarity to known sequences was determined by BLAST searches of the GenBank database. The names and accession numbers of significantly similar sequences are given. Expected values (E) of similarity are shown in parentheses.