Fig. 2. Characterization of polarized Raw 264.7 macrophages. (A) Immunofluorescence staining of M1 and M2 macrophages. Cells were stained with anti-CD38 antibodies (green) and anti-iNOS antibodies (red) for M1, anti-CD206 antibodies (green) and anti-Arg-1 antibodies (red) for M2. Scale bar is 20 μm. (B) Quantification of iNOS and CD38 expression on M1 macrophages, and Arg-1 and CD206 expression on M2 macrophages (****P < 0.0001). (C) Quantification of the size of M1 and M2 macrophages by ImageJ (n = 17).