Fig. 4. Raw 264.7 macrophage polarization with nanoghosts: (A) immunofluorescence staining of macrophages with anti-CD206 antibodies (green) and anti-Arg-1 antibodies (red). The arrows indicate reprogrammed macrophages. Scale bar is 20 μm. (B) Quantification of fluorescence signals related to Arg-1 and CD206 expression on macrophages in A (***P < 0.001, ****P < 0.0001). FACS analysis of CD206 positive ones in (C) M0 macrophages, (D) M2 macrophages polarized with the conventional method, and (E) M2 macrophages programmed by M2NGs.
