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. 2022 Aug 27;22:928. doi: 10.1186/s12885-022-09926-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Neuroendocrine differentiated colon cancer cells secrete exosomes. A, Fluorograms of stable neuroendocrine differentiated colon cancer line (LoVo-CgA) and negative control cell line (LoVo-NC), green fluorescent proteins (GFP) > 95%. Scale bar = 100 μm. B-C, The mRNA expression and protein expression of CgA was determined in LoVo-NC cells and LoVo-CgA cells using qRT–PCR assay (B) and western blot assay (C). D, The exosomes extracted from LoVo-NC cells (NC Exo) and LoVo-CgA cells (CgA Exo) showed typical cup shapes by transmission electron microscopy (TEM). Scale bar = 100 nm. E, The particle size of NC Exo and CgA Exo both ranges from 45 to150nm. F, NC Exo and CgA Exo both expressed exosomal labeled protein CD63, TSG101, Alix and HSPA8. ***, P < 0.001; NC, negative control; CgA, chromogranin A; Exo, exosomes