Fig. 5. ROS formation. Washed platelets (1.0 × 108 platelets mL−1), loaded with 10 μM DCFH-DA, were pre-incubated with saline, 10 μM GAD or 100 μM GA and then stimulated for 15 min with thrombin (a) or collagen (b) as indicated. At the end of incubation, samples were immediately analyzed by flow cytometry. Data are the mean ± SD of at least six experiments carried out in duplicate.
