Fig. 5. Contribution of hydrogen bonding to carbon dot modulation of hIAPP fibrillation. (A) ThT fluorescence curves recorded in Tris buffer (solid curves) and PB buffer (broken curves). hIAPP alone (black curves); hIAPP + Tyr-C-dots (concentration of 0.03 mg mL⁻¹; blue curves); hIAPP + Phe-C-dots (concentration of 0.03 mg mL⁻¹; red curves). (B) CD spectra of hIAPP alone (black spectra) and together with Tyr-C-dots (concentration of 0.05 mg mL⁻¹; blue spectra) recorded in Tris buffer or PB buffer. Each spectrum was acquired after 24 hour incubation of the peptide or peptide/C-dot mixture.