Table 1.

Comparison of different NGS technologies. Adapted from [36]

	Roche 454	Illumina/Solexa genome analyzer	PacBio SMRT	MinION
Company	Roche	Illumina	Pacific Biosciences	Oxford Nanopore Technologies (ONT)
Release year	2005	2006/7	2010	2014
Method	Sequencing by synthesis	Sequencing by synthesis	Sequencing by synthesis	Direct sequencing
Price	$13,700	Expensive—$ 49,000 to over a million	$695	$1000 with 500 disposable flow cells
Average reads	100–150 bp	75 bp highly accurate	10-25 Kb	The longest reads over 100 Kb