FIG. 4.

Time course of AFP1 production in S. tendae NP51(pK11) cultivated in CRM medium. (A) AFP1 (■) in the culture filtrate determined by agar diffusion assays with P. variotii as the test fungus compared to growth as determined by dry weight (●). (B) SDS-PAGE of culture filtrates from S. tendae NP51(pK11) and purified AFP1. Lanes 1 to 5, culture filtrate (40 μl) taken at time points (1 to 5) indicated by arrows in panel A; lane A, purified AFP1 (5 μg); lane M, molecular mass markers. Proteins were stained with Coomassie blue. AFP1 is marked by an arrow.